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. 2017 Mar 29;8(21):34687–34697. doi: 10.18632/oncotarget.16670

Figure 3. JAK1 S646P mutation drives IL-3-independent growth of BaF3 cells.

Figure 3

(A) The proliferation of transduced BaF3 cells after withdrawing IL-3 for 3 days was assayed by CCK-8. Data are expressed as mean ± s.d of at least 3 independent experiments. **P < 0.01. (B) The viability of transduced BaF3 cells after withdrawing IL-3 for 48 h was determined by trypan blue exclusion method at the indicated time points. (C) The survival curves of transduced BaF3 cells cultured in the absence of IL-3 for 6 days were determined by CCK-8. (D) Cell cycle histograms of transduced BaF3 cells were assayed by flow cytometry. The proportion of G1 or S/G2 phase is expressed as percentage of total cells.