Figure 4. JAK1 S646P mutation induces malignant proliferation of BaF3 cells in nude mice.
2 × 106 BaF3 cells transduced with vector, WT JAK1, or S646P mutant were intravenously inoculated into nude mice. (A) WBC counts were monitored at the indicated time points. (B) GFP-positive cells in peripheral blood were monitored by flow cytometry. (C) At the time of sacrifice, the morphological changes of livers and spleens were photographed. (D, E) The weights of livers (D) and spleens (E) were recorded. Data are expressed as the mean± s.d. *P < 0.05. (F) The bone marrow smears were stained with Wright and photographed (magnification: ×1000). Representative abnormal cells were marked with arrows. (G) The percentage of GFP-positive cells in bone marrow at the time of sacrificed was detected by flow cytometry. Data are expressed as the mean ± s.d. *P < 0.05.