Table 2. Observed responses of xenografts 12 days after treatment in the 23 factorial design with three independent parameters and their two levels (GLUT-1 AS-ODNs and HIF-1α AS-ODNs 100 μg or 0 μg, X-ray irradiation 10 Gy or 0 Gy).
G | A (μg) | B (μg) | C (Gy) | Tumor weightX (g) | AI (%)X | MVDX | Necrosis rate (%)X | ||||
---|---|---|---|---|---|---|---|---|---|---|---|
Hep-2 | Tu212 | Hep-2 | Tu212 | Hep-2 | Tu212 | Hep-2 | Tu212 | ||||
G1 | 100 | 100 | 10 | 0.14 ± 0.02 | 0.25 ± 0.04 | 14.33 ± 0.67 | 27.24 ± 0.36 | 2.33 ± 1.33 | 4.00 ± 0.57 | 56.67 ± 3.33 | 46.67 ± 6.66 |
G2 | 100 | 100 | 0 | 0.47± 0.02 | 0.21 ± 0.05 | 18.99 ± 1.13 | 17.19 ± 1.16 | 9.33 ± 0.33 | 10.00 ± 0.57 | 11.67 ± 3.33 | 36.67 ± 8.81 |
G3 | 0 | 100 | 10 | 0.42 ±0.12 | 0.30 ± 0.06 | 4.32 ± 0.03 | 6.42 ± 0.18 | 7.00 ± 1.00 | 7.66 ± 0.33 | 5.00 ± 0.00 | 40.00 ± 15.28 |
G4 | 100 | 0 | 10 | 0.26 ± 0.04 | 0.26 ± 0.05 | 4.82 ± 0.06 | 11.09 ± 0.30 | 16.67 ± 2.66 | 13.33 ± 0.88 | 10.00 ± 0.00 | 43.33 ± 8.18 |
G5 | 0 | 0 | 10 | 0.24 ± 0.04 | 0.33 ± 0.06 | 2.84 ± 0.15 | 3.88 ± 0.57 | 10.33 ± 1.20 | 14.00 ± 0.57 | 5.00 ± 0.00 | 23.33 ± 8.33 |
G6 | 0 | 100 | 0 | 0.53 ± 0.22 | 0.30 ±0.01 | 4.03 ± 0.18 | 5.78 ± 0.98 | 15.33 ± 0.88 | 16.00 ± 0.57 | 11.67 ± 3.33 | 16.67 ± 3.33 |
G7 | 100 | 0 | 0 | 0.60 ± 0.20 | 0.18 ± 0.04 | 8.24 ± 0.35 | 9.03 ± 0.49 | 25.33 ± 2.40 | 27.67 ± 1.45 | 5.00 ± 0.00 | 11.67 ± 1.66 |
G8 | 0 | 0 | 0 | 0.59 ± 0.02 | 0.42 ± 0.06 | 1.78 ± 0.21 | 2.73 ± 0.71 | 41.33 ± 1.45 | 45.00 ± 2.88 | 5.00 ± 0.00 | 5.00 ± 0.00 |
a (p-value) | 0.952 | 0.006 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 1.000 | |||
b (p-value) | 0.592 | 0.379 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 0.043 | |||
c (p-value) | 0.022 | 0.837 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 1.000 | |||
ab (p-value) | 0.051 | 0.292 | < 0.001 | < 0.001 | < 0.001 | 0.032 | < 0.001 | 0.018 | |||
ac (p-value) | 0.134 | 0.140 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 0.205 | |||
bc (p-value) | 0.873 | 0.767 | 0.173 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 0.162 | |||
abc (p-value) | 0.826 | 0.356 | 0.747 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 0.041 |
G: group, A: GLUT-1 AS-ODNs, B: HIF-1α AS-ODNs, C: X-ray irradiation.
a: main effect of GLUT-1 AS-ONDs, b: main effect of HIF-1α AS-ODNs, c: main effect of X-ray irradiation, ab: interaction effect between GLUT-1 AS-ONDs and HIF-1α AS-ODNs, ac: interaction effect between GLUT-1 AS-ONDs and X-ray irradiation, bc: interaction effect between HIF-1α AS-ODNs and X-ray irradiation, abc: interaction effect between GLUT-1 AS-ONDs and HIF-1α AS-ODNs and X-ray irradiation.
X Mean ± Std(standard deviation).