Table 4. Observed GLUT-1 and HIF-1α expressions of xenografts 12 days after treatment in the 2³ factorial design with three independent parameters and their two levels (GLUT-1 AS-ODNs and HIF-1α AS-ODNs 100 μg or 0 μg, X-ray irradiation 10 Gy or 0 Gy).

The results detected 12 days after treatment of xenografts
G	A (μg)	B (μg)	C (Gy)	GLUT-1mRNAX		Glut-1 proteinX		HIF-1α mRNAX		HIF-1α proteinX
				Hep-2	Tu212	Hep-2	Tu212	Hep-2	Tu212	Hep-2	Tu212
G1	100	100	10	0.51 ± 0.02	0.20 ± 0.04	0.54 ± 0.08	0.04 ± 0.01	0.14± 0.01	0.26 ± 0.03	0.07 ± 0.02	0.03 ± 0.00
G2	100	100	0	0.62 ± 0.03	0.36 ± 0.02	0.82 ± 0.01	0.17 ± 0.02	0.31 ± 0.01	0.38 ± 0.01	0.19 ± 0.08	0.05 ± 0.01
G3	0	100	10	0.33 ± 0.02	0.59 ± 0.09	0.55 ± 0.15	0.26 ± 0.06	0.40 ± 0.03	0.34 ± 0.04	0.18 ± 0.06	0.09 ± 0.03
G4	100	0	10	0.50 ± 0.05	0.31 ± 0.04	0.76 ± 0.02	0.13 ± 0.02	0.34 ± 0.03	0.59 ± 0.08	0.32 ± 0.02	0.32 ± 0.13
G5	0	0	10	0.80 ± 0.06	0.44 ± 0.06	0.59 ± 0.18	0.57 ± 0.15	0.65 ± 0.08	0.54 ±0.01	0.42 ± 0.08	0.29 ± 0.07
G6	0	100	0	0.31 ± 0.03	0.50 ± 0.04	0.59 ±0.12	0.48 ± 0.08	0.21 ± 0.02	0.50 ± 0.02	0.28 ± 0.08	0.12 ± 0.00
G7	100	0	0	0.78 ± 0.05	0.48 ± 0.11	0.94 ± 0.10	0.39 ± 0.11	0.83 ± 0.04	1.01 ± 0.12	0.64± 0.15	0.29 ± 0.05
G8	0	0	0	1.00 ± 0.07	1.01 ± 0.11	1.10 ± 0.20	1.40 ± 0.39	1.00 ± 0.05	1.01 ± 0.11	0.66 ± 0.04	0.54 ± 0.15
a (p-value)				0.023	< 0.001	0.506	0.001	< 0.001	0.515	0.289	0.142
b (p-value)				< 0.001	0.01	0.036	0.004	< 0.001	< 0.001	< 0.001	0.001
c (p-value)				0.02	0.001	0.017	0.006	< 0.001	< 0.001	0.014	0.006
ab (p-value)				< 0.001	0.049	0.618	0.058	0.021	0.200	0.754	0.69
ac (p-value)				0.011	0.05	0.874	0.1570	0.001	0.623	0.727	0.217
bc (p-value)				0.001	0.006	0.386	0.1221	< 0.001	0.004	0.244	0.473
abc (p-value)				0.001	< 0.01	0.164	0.305	0.107	0.936	0.793	0.228

G: group, A: GLUT-1 AS-ODNs, B: HIF-1α AS-ODNs, C: X-ray irradiation.

a: main effect of GLUT-1 AS-ONDs, b: main effect of HIF-1α AS-ODNs, c: main effect of X-ray irradiation, ab: interaction effect between GLUT-1 AS-ONDs and HIF-1α AS-ODNs, ac: interaction effect between GLUT-1 AS-ONDs and X-ray irradiation, bc: interaction effect between HIF-1α AS-ODNs and X-ray irradiation, abc: interaction effect between GLUT-1 AS-ONDs and HIF-1α AS-ODNs and X-ray irradiation.

^X Mean ± Std(standard deviation).