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. 2017 Apr 4;8(21):34867–34883. doi: 10.18632/oncotarget.16802

Figure 4. MiR-509-3-5P suppressed expression of PODXL via directly binding to its 3′UTR.

Figure 4

(A) There were two potential binding sites of miR-509-3-5P at the 3′-UTR of PODXL mRNA based on the bioinformatic analysis. 3′-UTR of PODXL mRNA containing wild type, mutant-1, mutant-2, mutant-both of was cloned into dual luciferase plasmid. (B1–B2) The mRNA expression of PODXL in BGC823 transfected with none, miR-509-3-5P/NC, miR-509-3-5P mimics and MGC803 transfected with none, miR-509-3-5P/NC, miR-509-3-5P/inhibitor. (C) Wild type (WT) or mutant-1(mut-1) or mutant-2 or mutant-both PODXL 3′-UTR was transfected into MGC803 with miR-509-3-5P/NC or miR-509-3-5P mimics or miR-509-3-5P/inhibitor, respectively. Firefly and Renilla luciferase signals were performed for luciferase activity after 36 h transfection. (D1–D2) The expression of PODXL in BGC823, MGC803 transfected with none, miR-509-3-5P/NC and miR-509-3-5P mimics or miR-509-3-5P/inhibitor, was measured by Western blot after 48 h transfection. Mean ± SEM was shown for these data.