Table 1. Identification of E. tenella>, E. acervulina and E. maxima sporozoite proteins in NCBI and Uniprot database using data from MALDI-TOF-MS/MS analyses.
| Spot IDa | Identified protein | Database ID |
|---|---|---|
| A929 | 14 kDa phosphohistidine phosphatase, putative (E. acervulina) | CDI76382.1 |
| M1436 | 14 kDa phosphohistidine phosphatase, putative (E. maxima) | CDJ61236.1 |
| T1468, T669 | 14 kDa phosphohistidine phosphatase, putative (E. tenella) | CDJ40270.1, U6KXP8|U6KXP8 |
| T1475 | 14-3-3 protein (E. tenella) | gi|21541950 |
| A1222, A1392, A1395 | 14-3-3 protein, putative (E. acervulina) | XP_013250285 |
| M567 | 14-3-3 protein, putative (E. maxima) | XP_013335589 |
| M1056, M1064, M1414 | 56 kDa gametocyte antigen (E. maxima) | gi|25989587 |
| T1064 | 56 kDa gametocyte antigen, related (E. tenella) | CDJ41536.1 |
| A620 | 56 kDa gametocyte antigen, related OS= E. acervulina | CDI78697.1 |
| M1131 | 82 kDa gametocyte antigen OS= E. maxima | Q86LH7|Q86LH7 |
| T1472 | Actin depolymerizing factor (E. tenella) | ABM89551.1 |
| A1510 | Actin depolymerizing factor, putative (E. acervulina) | CDI83856.1 |
| M1647, M1343 | Actin depolymerizing factor, putative (E. maxima) | CDJ60866.1 |
| M469 | Actin, putative OS=E. maxima | U6M655|U6M655_ |
| T941 | Alanine dehydrogenase, putative (E. tenella) | CDJ43978.1 |
| A604 | Alanine dehydrogenase, putative OS=E. acervulina | U6GM75|U6GM75 |
| A1448 | Aldo/keto reductase family oxidoreductase, putative (E. acervulina) | CDI80286.1 |
| T1145 | Aldo/keto reductase family oxidoreductase, putative (E. tenella) | CDJ45355.1 |
| M584 | Aldo/keto reductase family oxidoreductase, putative OS=E. maxima | CDJ57423.1 |
| T393 | Aspartyl proteinase (Eimepsin) OS=E. tenella | Q9GN67|Q9GN67 |
| A1458 | Cytosol aminopeptidase, putative (E. acervulina) | CDI77668.1 |
| T362 | Cytosol aminopeptidase, putative OS=E. tenella | U6KUY7|U6KUY7 |
| M1619, M1641 | Dihydrolipoyl dehydrogenase OS=E. maxima | CDJ55972.1 |
| A1368 | Dihydrolipoyl dehydrogenase, putative (E. acervulina) | CDI82910.1 |
| T1376 | Dihydrolipoyl dehydrogenase, putative (E. tenella) | CDJ38931.1 |
| T415 | Dynein heavy chain protein, related OS=E. tenella | U6KWQ6|U6KWQ |
| A1970 | Elongation factor 1-alpha OS=E. acervulina | U6GWZ2|U6GWZ |
| M480, M470 | Elongation factor 1-alpha, putative (E. maxima) | gi|557198794 |
| M478 | Elongation factor 2, putative (E. maxima) | gi|557157066 |
| T811 | Elongation factor 2, putative (E. tenella) | gi|557140071 |
| A939, A965 | Elongation factor 2, putative OS=E. acervulina | U6GRG2|U6GRG2 |
| T747 | Enolase 2, putative (E. tenella) | CDJ38513.1 |
| A1456 | Enolase 2, putative OS=E. acervulina | CDI82390.1 |
| M1548 | Enolase 2, putative OS=E. maxima | CDJ56218.1 |
| A744, A742 | Fructose-bisphosphate aldolase OS=E. acervulina | CDI80998.1 |
| T398 | Fructose-bisphosphate aldolase OS=E. tenella | CDJ43237.1 |
| M1060 | Fructose-bisphosphate aldolase, related (E. maxima) | CDJ59017.1 |
| A751, A748 | Glyceraldehyde-3-phosphate dehydrogenase OS=E. acervulina | CDI78463.1 |
| T403 | Glyceraldehyde-3-phosphate dehydrogenase OS=E. tenella | CDJ43289.1 |
| M1249 | Glyceraldehyde-3-phosphate dehydrogenase, putative (E. maxima) | CDJ57266.1 |
| A1581, A1340 | Haloacid dehalogenase-like hydrolase domain-containing protein, putative (E. acervulina) | CDI76904.1 |
| T1287 | Haloacid dehalogenase-like hydrolase domain-containing protein, putative (E. tenella) | CDJ37571.1 |
| M471 | Haloacid dehalogenase-like hydrolase OS=E. maxima | CDJ61159.1 |
| T1185 | Hypothetical protein (E. tenella) | gi|357017711 |
| T1316 | Hypothetical protein (E. tenella) | AET50460.1 |
| T1447 | Hypothetical protein (E. tenella) | AET50635.1 |
| T1208 | Hypothetical protein (E. tenella) | gi|357017711 |
| A841 | Hypothetical protein, conserved (E. acervulina) | CDI78255.1 |
| T1263 | Hypothetical protein, conserved (E. tenella) | CDJ37254.1 |
| M1375 | KH domain-containing protein, putative (E. maxima) | CDJ59518.1 |
| T776 | KH domain-containing protein, putative (E. tenella) | CDJ38027.1 |
| A895 | KH domain-containing protein, putative OS=E. acervulina | CDI84033.1 |
| A1618 | Lactate dehydrogenase (E. acervulina) | ACM77785.1 |
| M783, M762 | Lactate dehydrogenase OS=E. maxima GN=LDH | Q8I8U3|Q8I8U3_E |
| T718 | Lactate dehydrogenase OS=E. tenella | CDJ37067.1 |
| A1736 | Microneme 2 (E. acervulina) | KR063282.1 |
| M1002 | Microneme protein 7 OS=E. maxima GN=mic7 | G0LEU8|G0LEU8 |
| T1502 | Microneme protein MIC3, partial (E. tenella) | gi|40549149 |
| T1449 | Mitochondrial branched-chain alpha-keto acid dehydrogenase E1, putative (E. tenella) | CDJ43491.1 |
| A626 | Mitochondrial branched-chain alpha-keto acid dehydrogenase E1, putative, partial (E. acervulina) | CDI81447.1 |
| T692 | Nucleoside diphosphate kinase OS=E. tenella | U6KIW7|U6KIW7 |
| M373 | Nucleoside diphosphate kinase, putative (E. maxima) | CDJ59193.1 |
| A927 | Peroxiredoxin, putative (E. acervulina) | CDI84011.1 |
| M1109 | Peroxiredoxin, putative OS=E. maxima | CDJ59087.1 |
| T360 | Peroxisomal catalase, putative OS=E. tenella | CDJ43752.1 |
| A1233, A922 | Proteasome subunit alpha type 7, putative (E. acervulina) | CDI79975.1, U6GIG8|U6GIG8_ |
| T893, T624 | Proteasome subunit alpha type 7, putative (E. tenella) | CDJ41908, U6KYA5|U6KYA5 |
| M475, M476, M434, M875 | Purine nucleoside phosphorylase, putative (E. maxima) | CDJ57289.1 |
| T1083 | Purine nucleoside phosphorylase, putative (E. tenella) | CDJ44020.1 |
| A996 | Purine nucleoside phosphorylase, putative OS=E. acervulina | CDI78710.1 |
| T533 | Putative uncharacterized protein OS=E. tenella | H9B9×1|H9B9×1_ |
| A603, A614, A618 | Pyruvate kinase, putative (E. acervulina) | CDI78351.1 |
| A1326 | Sporozoite antigen, partial (E. acervulina) | CAA33905.1 |
| T623 | Transhydrogenase (Fragment) OS=E. tenella | Q24937|Q24937 |
| T714 | Transhydrogenase OS=E. tenella GN=7B2 PE=4 | Q07600|Q07600 |
| A1078, A1717 | Transhydrogenase, putative (E. acervulina) | CDI76761.1 |
| M1718 | Transhydrogenase, putative (E. maxima) | CDJ61620.1 |
| T1028, T665 | Triosephosphate isomerase (E. tenella) | CDJ37485.1, H9BA04|H9BA04 |
| A958, A1772 | Triosephosphate isomerase OS=E. acervulina | U6GH90|U6GH90, CDI79606.1 |
| M1606, M1212 | Triosephosphate isomerase, putative (E. maxima) | CDJ60494.1 |
| A1481, A1438, A1452 | Ubiquitin-conjugating enzyme domain-containing protein, putative (E. acervulina) | gi|557117367 |
| M699 | Ubiquitin-conjugating enzyme domain-containing protein, putative (E. maxima) | CDJ61561 |
| T1063 | Ubiquitin-conjugating enzyme domain-containing protein, putative (E. tenella) | XP_013236351 |
| M995, M909 | Uncharacterized protein OS=E. maxima | U6M744|U6M744_ |
| M858 | Uncharacterized protein OS=E. maxima | U6LYQ2|U6LYQ2 |
Note: 1.a Number of the spot in the 2-DE gel and the western blot membrane; 2. A: E. acervulina, T: E. tenella, M: E. maxima.