Table 1. Development of SCNT embryos derived from donor cells with Dnmt1s knockdown.
Group | No. embryoscultured (Rep.) | No. embryos cleaved(% ± SEM) | No. blastocysts(% ± SEM) | Blastocystcell numbers(mean ± SEM) * |
---|---|---|---|---|
control | 163 (5) | 138 (84.75 ± 1.98) | 33 (20.37 ± 1.15)a | 35 ± 2 (n=32) |
siRNA- negative | 167 (5) | 139 (83.23 ± 1.43) | 36 (21.50 ± 1.06)a | 34 ± 3 (n=34) |
siRNA- positive | 176 (5) | 153 (87.28 ± 1.73) | 55 (31.33 ± 1.49)b | 38 ± 3 (n=29) |
*Blastocyst cell numbers, less than 16, were not included, and 25 blastocysts in the siRNA-positive group were used for molecular analysis.
a-bValues in the same column with different superscripts differ significantly (P < 0.05).