Figure 1. Mutation of a single allele of the cancer susceptibility gene BRCA1 leads to activation of RANKL expression in normal-like breast epithelial cells.

Top. Inactivating mutation (185delAG) of a single BRCA1 allele leads to haploinsufficiency, which results in genomic instability in the spontaneously immortalized MCF10A cell line. Bottom. Total RNA from BRCA1^+/+ and BRCA1^mut/+ MCF10A isogenic cell pairs was characterized in technical triplicates for the abundance of RANK (TNFRSF11A, Hs00921372_m1) and RANKL (TNFSF11, Hs00243522_m1) relative to housekeeping genes GADPH (Hs99999905_m1) and 18S (Hs99999901_s1). The transcript abundance was calculated using the delta Ct method and presented as relative quantification (RQ). *p<0.05.