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. 2017 Apr 11;8(21):35061–35068. doi: 10.18632/oncotarget.17043

Figure 4. Irradiation sensitivity is altered with Gαi3 mutation in A172 cells.

Figure 4

Western blotting assay results showed expression of Gαi3 (endogenous and mutant) in stable A172 cells with the dominant-negative Gαi3 (G202T, “DN-Gαi3”), the constitutively-active Gαi3 (Q204L, “CA-Gαi3”) or the empty vector (pGCL-GFP-puro, “GFP”) (A). Cells were also treated with irradiation (5 Gy) and cultured for indicated time; Cell death (MTT OD reduction, (B)) and apoptosis (Histone DNA ELISA assay, (C)) were tested. *p < 0.05.