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. 2017 Apr 11;8(21):35061–35068. doi: 10.18632/oncotarget.17043

Figure 5. Irradiation induces Gαi3 nuclear translocation and association with DNA-PKcs.

Figure 5

A172 cells were treated with irradiation (5 Gy) for 2 and 4 hours, nuclear fractions were isolated, expression of listed protein was tested (A), Lamin-B is the nuclear marker protein). The association between DNA-PKcs and Gαi3 in cell nuclei was tested by Co-IP assay (B). Stable A172 cells with scramble control shRNA (“scr-shRNA”), Gαi3 shRNA (“-b”), the dominant-negative Gαi3 (G202T, DN-Gαi3) or the constitutively-active Gαi3 (Q204L, CA-Gαi3), as well as wild-type (WT) and Gαi3 knockout (KO) MEFs, were treated with irradiation (5 Gy) and cultured for 48 hours, DNA damage was tested by γ-H2AX FACS assay, and γ-H2AX-postive cell ratio was recorded (C–E). *p < 0.05 vs. “Mock”. # p < 0.05 vs. “Irradiation” of “scr-shRNA” A172 cells (C), “GFP” A172 cells (D) or WT MEFs (E).