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At 6 hours post transfection, the cells were infected with reovirus (5 MOI) and MTT assay was done at 48 hours. The graph represents the mean percent increase in reovirus' anti-cancer activity upon TLR3 siRNA down-regulation as compared to non-targeted siRNA determined by MTT Assay. The siRNA enhanced reovirus cell killing of HCT116 is improved significantly (p= 0.0067).
