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. 2017 Apr 4;8(21):35138–35153. doi: 10.18632/oncotarget.16812

Figure 7.

Figure 7

(a) & (b) H&E stained tumor photograph at 20X magnification of tumor generated by HCT116 upon reovirus treatment. No area of necrosis is observed. (c) & (d) H&E stained tumor photograph at 20X magnification of tumor generated by TLR3 silenced HCT116 cells followed by upon reovirus treatment. Distinct pink area of necrotic region is observed with islands of tumor cells demonstrating that the virus caused better dissemination of the tumor cells. (e) graphical representation of morphometric analysis to quantify the area of necrosis in reovirus treated xenografts. We documented that there is significant increase in necrosis both in reovirus treated HCT116 xenografts as well as HCT116-TLR3 KO xenografts when compared to untreated (p=0.0065 & p=0.0009) group. Furthermore, the extent of necrosis was significantly greater in HCT116-TLR3 KO when compared to HCT116 xenograft treated with reovirus.