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. 2017 Apr 7;8(21):35205–35221. doi: 10.18632/oncotarget.16925

Figure 5. In vitro autophagy inhibition reverses chemoresistance in cancer stem-cells derived from XBC4, the resistant TNBC xenograft model.

Figure 5

(A) Invalidation of BECN1 or BNIP3L genes with CRISPR-Cas9 technology in XBC4 spheres. Bar graphs show a decrease of 71% in BECN1 copy number in sg1BECN1 transfected cells (left panel), and a decrease of 65% in BNIP3L copy number in sg1BNIP3L cells (right panel). (B) The mean number of autophagosomes per 1000 μm<sup>2</sup> of cytoplasmic area on electron microscopy is lower in XBC4 spheres transfected with sg1BECN1 or with sg1BNIP3L than in XBC4 spheres transfected with empty plasmids. (C) Under normoxia, spheres from XBC4 transfected with sg1BECN1 or with sg1BNIP3L have a higher sensitivity to cisplatin than spheres transfected with empty plasmids. *p < 0.05.