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. 2005 Feb;73(2):1106–1115. doi: 10.1128/IAI.73.2.1106-1115.2005

FIG. 5.

FIG. 5.

Immunofluorescence microscopy analysis of decreased complement factor C3b deposition in 10% normal human serum by B. pseudomallei capsule. B. pseudomallei 1026b and SR1015 were incubated in 10% normal human serum (NHS), reacted with a mouse monoclonal antibody to human complement factor C3b, reacted with a rabbit anti-mouse IgG conjugated to Cy3 (Jackson Laboratories), and stained with DAPI for visualization of whole bacterial cells (Sigma). (A) B. pseudomallei 1026b incubated in PBS; (B) 1026b incubated in 10% NHS for 15 min; (C) 1026b incubated in 10% NHS for 60 min; (D) B. pseudomallei SR1015 incubated in PBS; (E) SR1015 incubated in 10% NHS for 15 min; (F) SR1015 incubated in 10% NHS for 60 min. The blue fluorescence indicates the DAPI-stained bacteria, and the red fluorescence indicates the binding of complement factor C3b to the bacterial surface.