TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araΔ139 Δ(ara-leu)7697 galU galK λ− rpsL nupG λ−tonA | Invitrogen |
| HB101 | F− Δ(gpt-proA)62 leu supE44 ara14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Smr) xyl-5 mtl-1 recA13 | 3 |
| B. pseudomallei | ||
| 1026b | Clinical isolate; Kmr Gmr Smr Pmr Tcs Tps | 11 |
| DD503 | 1026b derivative; allelic-exchange strain; Δ(amrR-oprA) (Kms Gms Sms) rpsL (Smr) | 21 |
| SR1015 | 1026b(pSR1015); Smr Tcr | 25 |
| SLR5 | SR1001 derivative; wcbB::Tn5-OT182 Tcr | 25 |
| SZ210 | DD503 derivative; ΔwcbB | This study |
| SZ210(pSZ219) | SZ210 derivative; pSZ219; Kmr | This study |
| SZ211 | DD503 derivative; pML102; Gmr | This study |
| SZ213 | DD503 derivative; pSZ213; Gmr | This study |
| B. thailandensis | ||
| E264 | Soil isolate; Kmr Gmr Smr Pmr Tcs Tps | 5 |
| Plasmids | ||
| pGSV3-lux | Mobilizable suicide vector containing lux operon from P. luminescens; OriT; Gmr | 22 |
| pBHR1 | Broad-host-range mobilizable cloning vector; Cmr Kmr | MoBiTec |
| pBluescript SK | General cloning vector; ColEI; Apr | Stratagene |
| pKAS46 | Mobilizable allelio-exchange vector; π-dependant R6K replicon; Apr KmrrpsL+ (Sms) | 28 |
| pCR2.1-TOPO | Topoisomerase-mediated cloning vector; Apr Kmr | Invitrogen |
| pRK2013 | Self-transmissible helper plasmid; Kmr | 15 |
| pSZ110 | 1.2-kb BamHI-KpnI fragment containing 600-bp upstream and downstream fragments of wcbB cloned into pBluescript SK; ΔwcbB; Apr | This study |
| pSZ210 | 1.2-kb XbaI-KpnI fragment containing 600-bp upstrcam and downstream fragments of wcbB cloned into pKAS46; ΔwcbB; Apr Kmr | This study |
| pML102 | 670-bp internal region of wcbB gene cloned as an EcoRI fragment into pGSV3-lux; Gmr | This study |
| pSZ212 | 589-bp internal region of wbiA gene cloned into pCR2.1-TOPO; Apr Kmr | This study |
| pSZ213 | 589-bp internal region of wbiA gene cloned as an EcoRI fragment into pGSV3-lux; Gmr | This study |
| pSZ219 | 1.5-kb wcbB gene and promoter region cloned as an NcoI-PinAI fragment into pBHR1; Kmr | This study |