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. 2005 Feb;73(2):1106–1115. doi: 10.1128/IAI.73.2.1106-1115.2005

TABLE 2.

Sequence of oligodeoxyribonucleotide primers used in this study

Primer name Sequencea
UpwcbBBamHI(+) 5′-GCG GGA TCC GCG CGC CAC TGG CCC CCG AC-3′
WcbBstartHindIII(−) 5′-GCC AGA AGC TTG TAA TAG CGT GGA AAT TCC ATT CCG CTG TGG CTT G-3′
WcbBendHindIII(+) 5′-GCC AAG CTT GAC ACG CTG CGC TAA ATG ACC CGA TCC ACC-3′
DownwcbBKpnI(−) 5′-GCC GGT ACC ACG ATC TCT CGT GCG GGC GAG-3′
WcbB-F2 5′-GCC AAT TCC CGA TCG ACG CTG GAA T-3′
WcbB-R2 5′-GCA GCG TGT CAA GAA AGG CAT CGA-3′
WbiA-int-F 5′-GCT TTC TCA TTG CGA AGA GCG G-3′
WbiA-int-R 5′-GCA AAC GAC GAC AGC AAA CGC T-3′
WcbBfull3NcoI(+) 5′-GCC CAT GGT GAC ACA CGC GAT CAA AAA CGT-3′
WcbBfull3PinAI(−) 5′-CGA CCG GTA TGC AGT AAG GTG TTT GAC CA-3′
a

Underlining indicates the restriction enzyme recognition site engineered into the primer.