TABLE 2.
Primer name | Sequencea |
---|---|
UpwcbBBamHI(+) | 5′-GCG GGA TCC GCG CGC CAC TGG CCC CCG AC-3′ |
WcbBstartHindIII(−) | 5′-GCC AGA AGC TTG TAA TAG CGT GGA AAT TCC ATT CCG CTG TGG CTT G-3′ |
WcbBendHindIII(+) | 5′-GCC AAG CTT GAC ACG CTG CGC TAA ATG ACC CGA TCC ACC-3′ |
DownwcbBKpnI(−) | 5′-GCC GGT ACC ACG ATC TCT CGT GCG GGC GAG-3′ |
WcbB-F2 | 5′-GCC AAT TCC CGA TCG ACG CTG GAA T-3′ |
WcbB-R2 | 5′-GCA GCG TGT CAA GAA AGG CAT CGA-3′ |
WbiA-int-F | 5′-GCT TTC TCA TTG CGA AGA GCG G-3′ |
WbiA-int-R | 5′-GCA AAC GAC GAC AGC AAA CGC T-3′ |
WcbBfull3NcoI(+) | 5′-GCC CAT GGT GAC ACA CGC GAT CAA AAA CGT-3′ |
WcbBfull3PinAI(−) | 5′-CGA CCG GTA TGC AGT AAG GTG TTT GAC CA-3′ |
Underlining indicates the restriction enzyme recognition site engineered into the primer.