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CaNAT1 confers nourseothricin resistance on C. albicans. The ura3/ura3 strain CAI4 was transformed with the empty vector pAU34, which bears the selectable marker URA3, giving rise to JKC347 and JKC348, and with pAU34 containing CaNAT1, giving rise to JKC345 and JKC346. Transformants were streaked onto medium to select for uridine prototrophy (sc-ura). They were then replica plated onto YPD containing 250 μg of nourseothricin/ml (YPD+nat250). 1, JKC437; 2, JKC438; 3, JKC436; 4, JKC435.
