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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Eur J Nucl Med Mol Imaging. 2017 Mar 6;44(8):1296–1305. doi: 10.1007/s00259-017-3663-y

Fig. 1.

Fig. 1

Evaluation of binding affinity of pertuzumab to HER2. (A) Western blot analysis of HER2 expression in three ovarian cancer cell lines showed high expression in SKOV3 cells and low expression in OVCAR3 and Caov3 cells. (B) HER2 expression was examined using flow cytometry, revealing high HER2 expression of SKOV3 cells and low levels of HER2 expression by OVCAR3 and Caov3 cells. (C) Saturation binding assays showed that 64Cu-NOTA-pertuzumab displayed specific binding to SKOV3 cells, with a receptor density of 1.6 million HER2 receptors per SKOV3 cell.