Figure 1.

In oestrogen receptor (ER)-negative human breast tumours P70S6K phosphorylation correlates with SK1 and VEGF expression. Human breast tumours (n = 30) were stained with anti-SK1, anti-VEGF and anti-p-P70S6K antibodies. Tumour sections were immunostained as described in materials and methods and fluorescence was visualised using Zeiss Axioplan microscope and quantified using ImageJ software. (A) Representative immunofluorescence images of two different tumour sections. Correlation between VEGF and p-P70S6K (B) SK1 and p-P70S6K (C) and SK1 and VEGF (D) in FFPE human ER-negative breast tumours.