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. 2017 Jun 14;7:3493. doi: 10.1038/s41598-017-03728-3

Figure 2.

Figure 2

RAD001 but not docetaxel decreases SK1 expression in MDA-MB-231 and PC3 cells. MDA-MB-231 and PC3 cells were starved overnight then incubated with 0.1% DMSO (Cont), 100 nM RAD001 (RAD), 5 nM docetaxel (Doc) and the combination of these drugs (RAD + Doc) for 24 h. (A,D) P70S6K phosphorylation was measured using ELISA. (B,E) SK1 expression was determined by qRT-PCR. (C,F) SK1 activity was measured using radiolabelling. Columns, mean of three independent experiments performed in triplicate; bars, SEM. (*P < 0.05; **P < 0.01; §P < 0.001; ns, not significant, P > 0.05).