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. 2017 Jun 14;7:3451. doi: 10.1038/s41598-017-03759-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Synovial fluid from obese OA patients contains greater amounts of IL-6 and IL-6R than synovial fluid from normal-weight OA patients. (A) Bio-plex assay data showing the profile of inflammatory markers present in the synovial fluid of normal-weight (n = 8) and obese (n = 8) OA patients. (B) sIL-6R expression in n = 8 normal-weight (N) and n = 8 obese (O) OA synovial fluid samples was determined by Western blotting and densitometry loaded for equal total protein content (20 µg). O* represents an obese sample that was loaded onto both blots for standardisation. (C) Bio-plex assay data showing leptin concentration in the synovial fluid of normal-weight (n = 19) and obese (n = 27) OA patients. Data are mean ± SEM. Statistical significance was determined by Mann-Whitney U test. *p < 0.05, **p < 0.01, N/S = not significant.