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. 2017 Jun 14;7:3513. doi: 10.1038/s41598-017-03489-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the LB and DMEM RNA-Seq samples from all of the E. coli isolates. (A) Principal component (PC) analysis of the normalized expression values of the LB and DMEM samples for ETEC isolate H10407, non-pathogenic isolate HS, EPEC isolates E2348/69 and 402290, and the EPEC/ETEC hybrid isolates 102651, 102712, and 401140. Each of the RNA-Seq samples is indicated by a different color in the legend at the top of the panel. The squares represent the DMEM samples and the triangles represent the LB samples. The red line separates all of the LB and DMEM samples, and the blue circle identifies the samples of the LT+ EPEC/ETEC isolates 102651 and 102712. (B) Hierarchical clustering of 520 gene clusters of PC1 and (C) 263 gene clusters of PC2. The heatmaps in these two panels display the normalized gene expression values of the LS-BSR gene clusters that were present in all of the genomes and had the greatest deviation in their expression values.