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. 2017 Jun 14;7:3513. doi: 10.1038/s41598-017-03489-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential expression of E. coli virulence genes. (A) In silico detection of E. coli virulence genes in each of the E. coli genomes analyzed in this study using RNA-Seq. The colors of the heatmap represent the BSR values of each gene with yellow indicating a gene is present and blue indicating a gene is absent. (B) Heatmap of the differential expression of the virulence genes for each of the sample comparisons of the E. coli isolates. Only significant log₂ fold-change (LFC) values ≥1 or ≤−1 are represented in the heatmap. Red indicates increased differential expression while green indicates decreased differential expression. White indicates that a gene was either not present in the E. coli isolate, and/or did not exhibit significant differential expression. The only sample comparison of EPEC isolate 402290 that is shown is for DMEM compared to LB since this isolate was analyzed previously and was not grown with added bile salts (Hazen et al. unpublished).