Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 14;7:3524. doi: 10.1038/s41598-017-01675-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Post-embryonic nmy-2 knockdown results in progressive seam cell loss following asymmetric divisions. (a) Combining the ts allele with RNAi was necessary to achieve a decrease of terminal seam nuclei numbers. Synchronised L1 larvae (hatched from animals grown at 15 °C) with or without the nmy-2(ne3409) or nmy-2(ne1490) ts mutations (strains AW335, AW785 and AW786, respectively) were fed control or nmy-2 RNAi at 25 °C. Seam nuclei were counted in young adults using the integrated seam cell marker wIs51[scmp::gfp]. The y-axis starts at 10 because the L1 larvae are born with 10 seam cells and gain additional seam cells through post-embryonic development. “WT” = wild-type. n > 50 for each sample. Error bars represent the standard error of the mean (SEM). Unpaired, two-tailed t-test was used to determine significance (****p < 0.0001). At least three independent replicates of the experiment were performed. Hashed pattern here and henceforth represents nmy-2 RNAi treatment. (b,c) Wild-type and nmy-2 knockdown adult hermaphrodites with the wIs51 seam cell marker. The wild-type animal showed 16 seam nuclei on one side, and the nmy-2 knockdown animal had 9. (d,e) Wild-type and nmy-2 knockdown adult hermaphrodites with the integrated membrane and DNA markers heIs63[wrt-2p::gfp::PH;wrt-2p::gfp::H2B]. The wild-type animal showed a smooth and continuous seam syncytium, and the nmy-2 knockdown animal showed two gaps in the seam syncytium (dashed lines). (f) The percentage of worms with at least one gap in the seam was quantified for the control and nmy-2 knockdown conditions following each seam cell division from L2 onwards (strains SV1009 and AW788). L2.1 = L2 first (symmetric) division. L2.2 = L2 second (asymmetric) division. n > 50 per strain per stage. Two independent replicates of the experiment were performed.