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. 2017 Jun 14;7:3492. doi: 10.1038/s41598-017-03698-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Membrane-tethered FtsZ2 forms multiple Z ring-like structures. (A) Fluorescence images of sfGFP-AtFtsZ2 tagged with 2MTS in E. coli WM1074 strain. Scale bar: 5 μm. (B) Filament morphology of sfGFP-AtFtsZ2 with or without 2MTS in the FtsZ-depleted strain WM2767. Scale bar: 5 μm. (C) Quantification of Z ring-like structures formed by sfGFP-AtFtsZ2 with or without 2MTS in the WM1074 and WM2767 strains. The graph shows the percentage of cells containing >1 Z-ring-like structure. The numbers of elongated bacterial cells investigated for the quantification were 212 (WM1074/sfGFP-AtFtsZ2), 123 (WM1074/sfGFP-AtFtsZ2-2MTS), 97 (WM2767/sfGFP-AtFtsZ2), and 41 (WM2767/sfGFP-AtFtsZ2-2MTS). (D) Concurrent observation of AtFtsZ2 Z ring-like structures and bacterial chromosomes in the WM2767 strain. Chromosomes were stained with DAPI. Scale bar: 5 μm.