Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 14;7:3461. doi: 10.1038/s41598-017-03561-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Role of the AT domain in recombinant EstPS1. (a) SDS-PAGE analysis of EstPS1 and EstPS1ΔAT. lane M - standard marker proteins; lane 1,2 - supernatant of cell lysate (EstPS1); lane 3 - purified protein of EstPS1; lane 4,5 - supernatant of cell lysate (EstPS1ΔAT); lane 6 - purified protein of EstPS1ΔAT. (b) SDS-PAGE analysis of EstPS1ΔSP and EstPS1ΔSP + AT. lane M - standard marker proteins; lane 7 - crude extracts of EstPS1ΔSP; lane 8 - purified protein of EstPS1ΔSP (inclusion bodies); lane 9 - crude extracts of EstPS1ΔSP + AT; lane 10 - purified protein of EstPS1ΔSP + AT (inclusion bodies). (c) Transparent zones of EstPS1 and EstPS1ΔAT in the solid induction medium at different hours. (d) Effect of the AT domain on secretion rate at 0 h, 8 h, 16 h, and 24 h. Secretion rate is defined as the enzyme activity of culture medium in proportion to the total activity. Data represent mean ± SD (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s-t test. (e) Comparison of the total activity (fermentation broth) between EstPS1 and EstPS1ΔAT with respect to the AT domain. (f) Residual activities of the enzymes after incubation at various temperatures (60–100 °C) for 10 min.