Table 2.

Substrate specificity of recombinant EstPS1.

Substrate	Specific activity (μmol min−1 mg−1)	Km (μM)	kcat (s−1)	kcat/Km (s−1 μM−1)
p-Nitrophenyl acetate	278 ± 21	102 ± 12	308 ± 23	3.02 ± 0.23
p-Nitrophenyl butyrate	425 ± 6	43 ± 8	481 + 7	11.19 ± 0.16
p-Nitrophenyl caproate	332 ± 12	76 ± 7	376 ± 14	4.95 ± 0.18
p-Nitrophenyl caprylate	376 ± 28	61 ± 10	417 ± 31	6.83 ± 0.51
p-Nitrophenyl decanoate	87 ± 5	362 ± 13	98 ± 6	0.271 ± 0.02
p-Nitrophenyl laurate	63 ± 7	391 ± 9	71 ± 8	0.182 ± 0.02
p-Nitrophenyl myristate	0	NM	NM	NM
p-Nitrophenyl palmitate	0	NM	NM	NM
Carbaryl	13.6 ± 0.86	0.21 ± 0.01	15.4 ± 0.98	73.3 ± 4.67
cis-Cypermethrin	6.5 ± 0.51	0.62 ± 0.031	7.4 ± 0.61	11.9 ± 0.98
trans-Cypermethrin	7.5 ± 0.58	0.53 ± 0.028	8.5 ± 0.66	16.1 ± 1.25
Fenpropathrin	12.8 ± 0.79	0.25 ± 0.014	14.5 ± 0.88	58 ± 3.52
Fenvalerate	4.3 ± 0.32	0.84 ± 0.042	4.9 ± 0.36	5.8 ± 0.43
Bifenthrin	0.92 ± 0.02	1.25 ± 0.061	1.1 ± 0.02	0.88 ± 0.02