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. 2017 Jun 14;7:3504. doi: 10.1038/s41598-017-03276-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In-vivo micro-SPECT/CT, ex-vivo BSGI imaging and semi-quantitative analysis. (a) Micro-SPECT/CT images (saggital) of untreated, treated ApoE^−/−, C57BL/6 J mice and ApoE^−/− mice with blocking at 4 h post injection of ^99mTc-MAG₃-bevacizumab, tracer accumulated area in thoracic aorta highlighted with oval white dotted line. (b) The semi-quantitative analysis results of micro-SPECT/CT scannings expressed as plaque-to-background ratio (P/B) for different parts of aortic plaque radioactivity uptake among different groups. (c) Ex vivo planar imaging of aorta by breast specific gamma imaging (BSGI). (d) The semi-quantitative analysis of mean value of plaque to non-plaque counts ratio (P/N) for BSGI imaging among untreated, treated ApoE^−/− and C57BL/6 J mice groups.