Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 14;7:3473. doi: 10.1038/s41598-017-03152-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

GATA6 regulation of AQP5 expression involves H3 acetylation. RNA and protein were harvested from MLE-15 cells transduced with lentivirus expressing Gata6 or control non-silencing shRNA (MOI = 2) for 3 days. qRT-PCR shows that knockdown of Gata6 (A) significantly decreases Aqp5 mRNA (B) and protein (AQP5 normalized to β-actin (42 kD)) (C,D) expression (n = 3, *p < 0.05 compared to non-silencing shRNA (NS-shRNA)). (E) ChIP with anti-acetyl-H3 Ab (H3Ac) demonstrates enrichment of H3 acetylation at the Aqp5 promoter/enhancer region homologous to the proximal 358-bp of the rat Aqp5 promoter using chromatin harvested from MLE-15 cells transduced with GATA6-expressing lentivirus or control (GFP) (MOI = 2) (n = 3, *p < 0.05). ChIP efficiency was calculated relative to control virus-transduced cells precipitated with H3Ac Ab, which was set as 1. Rabbit IgG pull-down is used as control.