Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 14;7:3473. doi: 10.1038/s41598-017-03152-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

p300 increases GATA6/Sp1-mediated -358-Aqp5-Luc activity. (A–C) NIH3T3 cells were co-transfected with -358-Aqp5-Luc and different combinations of GATA6, Sp1, p300 and CBP expression vectors. Luciferase assays were performed 48 h after transfection. p300 and CBP have no effect on Aqp5-Luc activity (A), while p300 (but not CBP) augments both GATA6- (B) and Sp1- (C) mediated Aqp5 transcriptional activity. Protein concentration was used to normalize samples for transfection efficiency (n = 3, *p < 0.05 compared to GATA6 or Sp1 only). qRT-PCR shows knockdown of p300 mRNA using two different shRNAs (V2LMN_102133 indicated as p300-1 and V2LMN_90586 indicated as p300-2) (D) decreases Aqp5 mRNA expression following p300 knockdown (E) (n = 4, *p < 0.05 compared to non-silencing shRNA control (NS shRNA)). WB shows knockdown of p300 (F,G) decreases AQP5 protein expression (F,H). (n = 4, *p < 0.05 compared to NS shRNA control).