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. 2017 Jun 14;7:3517. doi: 10.1038/s41598-017-03742-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hydrogen sulfide stimulates CFTR in Xenopus oocytes. (a) A representative current trace of a TEVC recording of a CFTR-expressing oocyte. The application of Na₂S (50 µM, black bar) as well as forskolin (fsk.; 5 µM) and IBMX (100 µM; grey bars) led to an increase in transmembrane current signals (I_M). (b) Statistical analysis of data obtained from experiments as shown in panel a. Depicted are values of I_M (before drug application or peak values after drug application) from individual experiments (grey symbols) as well as means ± SEM (*P ≤ 0.05, Wilcoxon signed rank test; **P ≤ 0.01, Student’s paired t-test). (c) Summarised data from experiments as similar to those shown in panels a and b, using native, non-CFTR-expressing oocytes. Values of I_M were taken at time point were CFTR-expressing oocytes of the same donor had the maximal response to the drugs. Depicted are means ± SEM (**P ≤ 0.01, Student’s paired t-test). (d) A representative current trace of a TEVC recording of a CFTR-expressing oocyte. After application of Na₂S (50 µM, black bar), the CFTR inhibitor CFTR_inh172 (CFTR_inh.; 25 µM) was additionally applied. This readily inhibited values of I_M. (e) Statistical analysis of data obtained from experiments as shown in panel d. Depicted are values of I_M (before drug application or peak values after drug application) from individual experiments (grey symbols) as well as means ± SEM (**P ≤ 0.01, Wilcoxon signed rank test). (f) Evaporative loss of H₂S was measured by monitoring the concentration of H₂S in the employed buffers solutions by the formation of methylene blue. Depicted are values for methylene blue absorbance at 670 nm over time. Na₂S (50 µM) exposure is indicated by the black bar. (g) A representative current trace of a TEVC recording of a CFTR-expressing oocyte. Both, GYY4137 (500 µM, grey bar) as well as Na₂S (50 µM, black bar) stimulated I_M. (h) Statistical analysis of data obtained from experiments as shown in panel g. Depicted are values of I_M (peak values after drug application) from individual experiments (grey symbols) as well as means ± SEM (*P ≤ 0.05, Wilcoxon signed rank test). Numbers of experiments (n) are indicated in parentheses.