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. 2017 Jun 14;7:3517. doi: 10.1038/s41598-017-03742-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H₂S stimulates CFTR via cAMP- and PKA-mediated signalling. (a) Representative current trace of a TEVC recordings of a CFTR-expressing oocyte. Transmembrane currents (I_M) were recorded and the oocyte was exposed twice to Na₂S (50 µM, black bar). (b) Statistical analysis of data obtained from experiments as shown in panel a. Depicted are values of the first (1) and second (2) Na₂S-induced current (I_Na2S) from individual experiments (grey symbols) as well as means ± SEM (n.s. = not significant, Student’s paired t-test). I_Na2S was calculated by subtracting the current before application of Na₂S from the peak value after application of Na₂S, resulting in positive values for I_NA2S. (c) Representative current traces of TEVC recordings of CFTR-expressing oocytes. Transmembrane currents (I_M) were recorded and oocytes were exposed twice to Na₂S (50 µM, black bar) DMSO (0.1%; left trace) or the AC inhibitor MDL 12330 A (MDL, 20 µM; right trace) were applied between the first and second stimulation with Na₂S (black arrowheads). (d) Statistical analysis of data obtained from experiments as shown in panel a. Depicted are values of the first (1) and second (2) Na₂S-induced current (I_Na2S) from individual experiments (grey symbols) as well as means ± SEM (**P ≤ 0.01, Student’s paired t-test). I_Na2S was calculated by subtracting the current before application of Na₂S from the peak value after application of Na₂S, resulting in positive values for I_NA2S. (e) Representative current traces of TEVC recordings of CFTR-expressing oocytes. Transmembrane currents (I_M) were recorded and oocytes were exposed twice to Na₂S (50 µM, black bar). The perfusion recording was stopped briefly between the first and second stimulation with Na₂S (grey lines). Then, an intracellular-analogous solution (IAS) or IAS containing the PK-antagonist cAMPS-Rp (87 µM) were injected into the oocytes before the second stimulation with Na₂S (black arrowheads). (f) Statistical analysis of data obtained from experiments as shown in panel a. Depicted are values of the first (1) and second (2) Na₂S-induced current (I_Na2S) from individual experiments (grey symbols) as well as means ± SEM (**P ≤ 0.01, Wilcoxon signed rank test). Numbers of experiments (n) are indicated in parentheses.