Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 14;7:3469. doi: 10.1038/s41598-017-02018-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Microgravity causes a decrease in β-catenin protein expression. (A) Semiquantitative–PCR analysis of growth factors GDNF and KL in Sertoli cells cultured for 48 h at G or under SM. (B) Western blot analysis of cytoskeletal proteins in extracts from Sertoli cells cultured for 48 h at G or under SM. Densitometric analysis of β-catenin is reported on the right showing a decrease in the protein level under SM. (C) Western blot analysis of β-catenin in extracts from Sertoli cells cultured for 48 h at G or under SM, in the presence or not of GSK-3β inhibitor CHIR 99021. Treatment inhibits degradation of β-catenin caused by SM. (D) Western blot analysis of β-catenin distribution in the nuclear (N) and cytosolic (C) fractions of Sertoli cells cultured for 48 h at G or under SM. At least three different experiments were performed. Bars represent s.d. Asterisks indicate: *P < 0.05.