Figure 4.

PBN does not affect P2X7R-induced Ca²⁺ increases. (A) BzATP induces intracellular Ca²⁺ increases through P2X7R activation in SCDH neurons. Left panel: Representative Ca²⁺ imaging recording of KCl- and BzATP-induced Ca²⁺ increases in the presence and absence of A438079. Right panel: Summary of the effects of A438079 on BzATP-induced calcium response. ( B ) PBN (30 µM) does not affect P2X7R function. Left panel: Representative Ca²⁺ imaging recordings of BzATP-induced Ca²⁺ increases in the presence and absence of PBN. Right panel: Summary of the effects of PBN on BzATP-induced calcium response. Values represent mean ± SEM, n = 15–16 neurons. ***P < 0.001 when compared to BzATP 300 µM by the unpaired Student’s t test.