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. 2017 Jun 14;7:3454. doi: 10.1038/s41598-017-03492-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro test result for complete mass reduction. (A) Required treatment time for 90% mass reduction of 200 mg clot samples with the treatment conditions of 80 V_pp input voltage, 10% duty cycle, and 10⁷ bubbles/ml injection with 100 μl/min flow rate (n = 3). The difference of treatment time between planar and concave prototypes showed no statistical significance. (B) A 200 mg clot sample decreased in volume and mass after 3.5 hr treatment (scale bar = 3 mm). The remaining small (~20 mg) fibrin (B2) was not completely removed by continued treatment (>4.5 hr).