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. 2017 Jun 14;7:3508. doi: 10.1038/s41598-017-03689-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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microRNA profile of CD4 T cells activated by cognate interaction with cDCs. CD4 T cells from OT-II mice were cocultured with cDCs in the presence or absence of OVA peptide for 18 h and their miRNA analyzed by miRNA microarray. (A) Comparison of miRNAs expression on T cells after stimulation by cDCs loaded or not with OVA peptide. (B) Selected miR-17-5p, miR-18a-5p, miR-132-3p, miR-26a-5p, miR-150-5p and miR-467b-5p miRNAs were validated by RT-qPCR. miRNAs that were detected by microarrays to be upregulated or downregulated are depicted in red and green respectively. (n = 8) (C) miRNA levels were assessed by RT-qPCR in freshly isolated mouse naive CD4 T cells in different time points of activation with ConA followed by IL-2. (Plots are representative of two independent experiments). RNU1A1 and RNU5G were used as endogenous controls and data are presented in arbitrary units (A.U.). ***P < 0.001; **P < 0.05; ns, non-significant.