Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 14;7:3508. doi: 10.1038/s41598-017-03689-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

miR-132-3p targets pik3r1. (A) 3′UTR of pik3r1 cloning strategy. Fragments from 71 to 362 bp and from 2957 to 3162 bp containing the two binding sites predicted for miR-132-3p were cloned into the psiCheck2 vector. (B) miR-132-3p levels in HEK cells after transfection. Levels were normalized to RNU1A1 and RNU5G. (C) HEK cells were transfected with indicated plasmids (Control empty vector or miR-132 expressing vector), GFP+ cells sorted and Renilla and Firefly luciferase signal measured. Data are presented in Renilla Luciferase signal relative to Firefly (n = 5). T-test **P < 0.05. (D) miR-132-3p expression in Jurkat cells 48 h post transfection with either negative Control-Dy547 or mmu-miR-132-3p miRNA Mimics. (E) Western blot analysis of p85α, p55α and p50α isoforms in Jurkat cells after 48 h of transfection with the control or miR-132-3p mimics as in (D). A representative blot of one experiment out of five is shown; protein bands were cropped from the same gel. (F) Protein relative levels of p85α, p55α and p50α as in (E) after normalization to p150 (n = 5) T-test **P < 0.05; *P < 0.01.