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. 2017 Jun 14;7:3486. doi: 10.1038/s41598-017-02358-z

Table 1.

Sequence of primers for semiquantitative and quantitative RT-PCR.

Gene Name Sequences (semiquantitative RT-PCR) Sequences (quantitative RT-PCR)
E-cadherin Forward 5′ -TGC TCT TGC TGT TTC TTC GG-3′ 5′-TGC TCT TGC TGT TTC TTC GG-3′
Reverse 5′-TGC CCC ATT CGT TCA AGT AG-3′ 5′-TGC CCC ATT CGT TCA AGT AG-3′
α-SMA Forward 5′-GGT GCT GTC TCT CTA GCC TCT GGA-3′ 5′-GGC TCT GGG CTC TGG GCT TCA TC-3′
Reverse 5′-CCC ATC AGG CAA CTC GAT ACT CTT C-3′ 5′-CTC TTG CTC TGG GCT TCA TC-3′
Vimentin Forward 5′-GAA GAG AAC TTT GCC GTT GAA G-3′ 5′-GAA GAG AAC TTT GCC GTT GAA G-3′
Reverse 5′-GAG AAA TCC TGC TCT CCT CG-3′ 5′-GAG AAA TCC TGC TCT CCT CG-3′
Fibronectin Forward 5′-GGA TGC TCC TGC TGT CAC-3′ 5′-CTT TGG TGC AGC ACA ACT TC-3′
Reverse 5′-CTG TTT GAT CTG GAC CTG CAG-3′ 5′-CCT CCT CGA GTC TGA ACC AA-3′
GAPDH Forward 5′-GTG GAT ATT GTT GCC ATC AAT GAC C-3′ 5′-GTG GAT ATT GTT GCC ATC AAT GAC C-3′
Reverse 5′-GCC CCA GCC TTC TTC ATG GTG GT-3′ 5′-GCC CCA GCC TTC TTC ATG GTG GT-3′
Snail Forward 5′-TCT AGG CCC TGG CTG CTA CAA-3′
Reverse 5′-GCC TGG CAC TGG TAC TTC AC-3′
Slug Forward 5′-ATG CAT ATT CGG ACC CAC C-3′
Reverse 5′-AGA TTT GAC CTG TCT GCA GCT C-3′