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. 2017 Jun 14;7:3448. doi: 10.1038/s41598-017-03449-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miRNA Profiling of miR-10b Transfected HCEC by Fluidigm QPCR. Transfection with miR-10b mimic using RNAiMax decreased significantly the mRNA expression level of some putative LESC markers and miR-10b predicted targets (a). Transfection with miR-10b inhibitor significantly increased their expression levels (b) in HCEC compared to their corresponding scrambled sequence controls (mimic control and inhibitor control, respectively). Further, the putative LESC marker mRNA levels that were increased in HCEC transfected with miR-10b, showed decreased levels in HCEC transfected with miR-10b inhibitor in comparison to corresponding controls. Student’s t-test P < 0.05.