FIGURE 7.

Quantification of tet genes in viromes and microbiomes isolated from Lambro River water-based mesocosms. Black bars refer to the C_t values obtained for quantification of tet genes in viromes isolated from tetracycline-exposed mesocosms. Gray bars refer to the C_t values obtained for quantification of tet genes in viromes isolated from control mesocosms. Dark gray bars refer to the C_t values obtained for quantification of tet genes in microbiomes isolated from tetracycline-exposed mesocosms. White bars refer to the C_t values obtained for quantification of tet genes in microbiomes isolated from control mesocosms. C_t values are expressed as the average of three independent qPCR assays ± the standard deviation. Statistically significant differences were determined by an unpaired Student’s t-test (^∗P < 0.05).