Fig. 5. The CLSM imaging of A375 cells treated with 3 μM TCNTP (A) and 3 μM Hoechst 33258 (B) for 4 h with further incubation for 1 d, 3 d and 5 d in cell culturing conditions. (C) The average fluorescence intensity of TCNTP and Hoechst 33258 in 60× microscopic field (the insets). (D) The number of living A375 cells in 10× microscopic field. The white scale bar: 100 μm; the red scale bar: 10 μm.
