Table 1. Data processing statistics for different complex states and model refinement.
| Presynaptic | Post-synaptic | Synaptic | |
|---|---|---|---|
| Data collection | |||
| TEM | Titan Krios | Titan Krios | Titan Krios |
| Camera | K2-Summit | K2-Summit | FalconII |
| Pixel size (Å) | 1.306 | 1.306 | 1.05 |
| Micrograph number | 540 | 528 | 202 |
| Image processing | |||
| Boxsize for S and R | 256 | 256 | 256 |
| Total particle number for S and R | 40,404 | 41,146 | 28,455 |
| Resolution (Å) | 4.4 | 4.5 | 5 |
| Boxsize for F | - | 320 | 400 |
| Total particle number for F | - | 2318 form 356 micrographs | 1998 |
| Good particle for F | - | 1072 | 552 |
| Resolution for F (Å) | - | 10 | 14 |
| Rise (Å) | 15.88 | 15.80 | 15.27 |
| Twist | 56.77° | 56.18° | 56.4° |
| Model building | |||
| Rms deviations (bonds) (Å2) | 0.0074 | 0.0098 | - |
| Rms deviations (angles) (Å2) | 1.03 | 1.13 | - |
| Molprobity score | 3.10 | 2.93 | - |
| Clashscore all atoms | 31.5 | 35.71 | - |
| Poor rotamers (%) | 4.59 | 3.49 | - |
| Ramachandran plot favored (%) | 91.89 | 92.07 | - |
| Ramachandran plot outliers (%) | 2.30 | 2.17 | - |
*
S stands for SPIDER, R stands for RELION and F stands for FREALIGN.