Table 1. Data processing statistics for different complex states and model refinement.
Presynaptic | Post-synaptic | Synaptic | |
---|---|---|---|
Data collection | |||
TEM | Titan Krios | Titan Krios | Titan Krios |
Camera | K2-Summit | K2-Summit | FalconII |
Pixel size (Å) | 1.306 | 1.306 | 1.05 |
Micrograph number | 540 | 528 | 202 |
Image processing | |||
Boxsize for S and R | 256 | 256 | 256 |
Total particle number for S and R | 40,404 | 41,146 | 28,455 |
Resolution (Å) | 4.4 | 4.5 | 5 |
Boxsize for F | - | 320 | 400 |
Total particle number for F | - | 2318 form 356 micrographs | 1998 |
Good particle for F | - | 1072 | 552 |
Resolution for F (Å) | - | 10 | 14 |
Rise (Å) | 15.88 | 15.80 | 15.27 |
Twist | 56.77° | 56.18° | 56.4° |
Model building | |||
Rms deviations (bonds) (Å2) | 0.0074 | 0.0098 | - |
Rms deviations (angles) (Å2) | 1.03 | 1.13 | - |
Molprobity score | 3.10 | 2.93 | - |
Clashscore all atoms | 31.5 | 35.71 | - |
Poor rotamers (%) | 4.59 | 3.49 | - |
Ramachandran plot favored (%) | 91.89 | 92.07 | - |
Ramachandran plot outliers (%) | 2.30 | 2.17 | - |
S stands for SPIDER, R stands for RELION and F stands for FREALIGN.