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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Cell Biol Int. 2016 Feb 15;40(4):478–483. doi: 10.1002/cbin.10578

Fig. 3. Characteristic structural features of muscle fibers are conserved after CLARITY treatment of EDL muscles while some modifications of fat cells and tendons are observed.

Fig. 3

A–B: 2P fluorescence shows characteristic striated autofluorescence in both control and treated fibers. The insets reproduce the areas marked with an asterisk. Nuclei are positioned, as expected, along plasma membrane and along blood vessels that appear as dark channels (arrows); (C–D): SHG imaging (red) and 2-photon autofluorescence (green) of tendon and fat cells (arrowheads) show that the former keep their basic organization while fat cells are depleted and flattened by the treatment; E–F: SHG imaging of unstained EDL fiber bundles shows conservation of the striated pattern. Bars: A–E: 50 µm (see bar in E); F: 25 µm; insets 10 µm (bar in A).