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. 2017 Mar 21;31(7):2881–2892. doi: 10.1096/fj.201601238R

Figure 3.

Figure 3.

LPS attenuates Nrp1 in macrophages. A, B) Quantitative RT-PCR analysis of Nrp1 mRNA in PMs (A) and BMDMs (B) treated with or without LPS (100 ng/ml) for 12 h. C, D) Immunoblot analysis of Nrp1 in PMs (C) and BMDMs (D) treated with or without LPS (100 ng/ml) for 12 h. E) Quantitative RT-PCR analysis of Nrp1 mRNA in RAW264.7 cells treated with LPS (100 ng/ml) for 0–24 h. F) Quantitative RT-PCR analysis of Nrp1 mRNA in RAW264.7 cells treated for 24 h with LPS (0–200 ng/ml). G) Immunoblot analysis of Nrp1 in RAW264.7 cells treated with LPS (100 ng/ml) for 0–24 h. H) Immunoblot analysis of Nrp1 in RAW264.7 cells treated for 24 h with LPS (0–200 ng/ml). β-Actin serves as loading control. Data are expressed as means ± sem. *P < 0.05 vs. control, 0 h, 0 ng/ml, or vehicle.