Figure 6.

Effect of Nrp1 deficiency on M2 polarization in BMDMs. Purified monocytes were incubated for 5 d with M-CSF (50 ng/ml) to differentiate into mature macrophages (M0-phenotype). M0-macrophages were then polarized for an additional 2 d into M2-macrophages by stimulation with IL-4 (20 ng/ml). A) Immunoblot analysis of ArgI and Ym1 in WT and Nrp1^−/− BMDMs. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) serves as loading control. B) Quantitative RT-PCR analysis of ArgI, Ym1, Ym2, IL-10, and CD206 mRNA levels in WT and Nrp1^−/− BMDMs. Data are expressed as means ± sem.