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. 2017 Jun 15;24:38. doi: 10.1186/s12929-017-0345-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The influence of NBP on mitochondrial function in H9c2 cardiomyoblasts exposed to H₂O₂. a The generation of ROS in different groups (Control group; H₂O₂ group; H₂O₂ + NBP group). b Micrographs of mitochondrial morphology by MitoTracker Red staining in H9c2 (Control group; H₂O₂ group; H₂O₂ + NBP group). c Mitochondrial membrane potential (MMP) was determined by evaluation of JC-1 staining under an upright fluorescence microscope after use of the JC-1 assay kit. d Cells were exposed to H₂O₂ for 2 h and then MMP was determined in the absence or presence of NBP. Results were expressed as the mean ± S.E.M. # P < 0.05 vs Control group; * P < 0.05 vs H₂O₂ group