Fig. 4.

Oct4/NEAT1 or Oct4/MALAT1 signaling axis promoted lung cancer cell proliferation and motility. a-d Cell proliferation assay. Cells were reconstituted with expression vector of NEAT1 (a) or MALAT1 (b) in Oct4-silenced A549 cells, or si-NEAT1 oligo (c) or si-MALAT1 oligo (d) in Oct4-overexpressed A549 cells. Treated cells were then re-seeded and analyzed by RealTime-Glo viability assay at indicated time points. e-h Transwell migration and invasion assays. (e, f). Cells were reconstituted with expression vector of NEAT1 or MALAT1 in Oct4-silenced A549 cells, or (g, h) si-NEAT1 oligo or si-MALAT1 oligo in Oct4-overexpressed A549 cells. Treated cells were re-seeded and analyzed by transwell migration and invasion assays. Results were photographed and quantified. Data represent mean ± SEM. P-values were determined by two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001