Fig. 1.

Verification of the mRNA expression levels of CypB gene identified from the forward SSH library by qRT-PCR. a According to qRT-PCR, the standard curve established showed an efficiency of 106.7% with R² of 0.991. b The mRNA expression levels of CypB gene in mock- and ORFV-infected MDBK cells at 0, 2, 4, 8, 12, 18 and 24 h post infection were measured by qRT-PCR. Statistical analysis was performed by Student’s t-test using GraphPad Prism 6 software. Values are the means ± SEM. (* P < 0.05, ** P < 0.01, *** P < 0.001). As shown in Fig. 1b, the transcript levels of CypB was overexpressed in MDBK cells infected with ORFV-Jilin (MOI = 3) compared with mock-infected MDBK cells, with CypB mRNA levels 10⁶-fold higher in ORFV-infected MDBK cells at 4 h post infection than in the corresponding mock-infected MDBK cells