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. 2017 Feb 15;1(2):108–114. doi: 10.1017/cts.2016.20

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© The Association for Clinical and Translational Science 2017

This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0/)which permits noncommercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work

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Fig. 1 — Representative flow cytometry plots: glucocorticoid receptor (GR) expression in granulocytes, monocytes, and T lymphocytes was determined by staining with GR FITC, CD16 Pacific Blue, CD14 PerCP, and CD3 APC, respectively, and identifying cells that were positive for both GR FITC and leukocyte subtypes of interest. (a) Forward-scattered (FSC) and side-scattered (SSC) plot of leukocytes; (b) histogram of unstained leukocytes (green), and after staining with IgG isotype-FITC (red) and GR FITC (blue); (c) double-positive signals for granulocytes CD16/GR, (d) monocytes CD14/GR, and (e) T lymphocytes CD3/GR, as identified in rectangular boxes.