Table 1.
Immunodominant bands recognized by Neospora caninum-infected or immunized animals in tachyzoite antigen
| Antigen treatment | Molecular weight in kDa of antigenic bands (antigen treatment)a | Source of N. caninum antibodies | Parasite species used to examine specificity | Reference |
|---|---|---|---|---|
| Non-reduced | 16, 29, 31, 32, 37, 46, 51, 56, 79 (SDS–PAGE sample buffer soluble total lysate) | Hyperimmune serum from a rabbit | Not examined | (Barta and Dubey, 1992) |
| 17, 29, 30, 37, 46, 88, 97 (aqueous buffer and detergent soluble total lysate) | Sera from several naturally and experimentally N. caninum infected (cattle, dog, sheep, goat, pig) or immunized animal species (rabbits) | T. gondii, S, cruzi, H. hammondi; faint reactions to 46 kDa observed in pre-immune serum of rabbits; 46, 88, 97 kDa antigens clearly and 30 kDa faintly and inconsistently recognized by T. gondii antiserum; no cross-reactivities reported with anti-S. cruzi or anti-H. hammondi | (Bjerkas et al. 1994) | |
| 17–18, 30–45, >51 (soluble part of sonicated total lysate and iscom incorporated antigen) | Serum from experimentally infected or immunized rabbits and from a naturally infected dogs | T. gondii, S. cruzi; anti-T. gondii showed faint reactions with 40 kDa; anti-S. cruzi: no reactions | (Björkman et al. 1994) | |
| 28–35, 45–52, 64–78 (ISCOM incorporated antigen) | Sera and milk from naturally and experimentally infected bovines | T. gondii; serum of a T. gondii-infected calf did not react | (Björkman et al. 1997) | |
| 28, 31, 40, 83, 91 (soluble part of sonicated total lysate) | T. gondii, S. cruzi; 40 kDa faintly stained by anti-T. gondii; anti-S. cruzi showed negligible reactions | (Stenlund et al. 1997) | ||
| 16/17, 29/30, 30–36, 37, 45 (SDS–PAGE sample buffer soluble total lysate) | Sera from experimentally infected calves and naturally infected cattle | T. gondii; no cross-reactions with anti-T. gondii | (Atkinson et al. 2000) | |
| 17, 29, 30, 33, 37, 40 (SDS–PAGE sample buffer soluble total lysate) | Sera from naturally infected bovine fetuses | Not examined | (Sondgen et al. 2001) | |
| 29, 36 (SDS–PAGE sample buffer soluble total lysate) | Sera from naturally infected bovines | T. gondii; no cross-reactions in 14 of 17 naturally seropositive cattle, faint reactions in the remaining (true infection status regarding N. caninum unknown) | (Staubli et al. 2006) | |
| 14, 29, 30, 33, 35, 40, 55, 60, 77, 84, 97, 170 (aqueous buffer and detergent soluble total lysate) | Sera from experimentally and naturally infected dogs | Not examined | (Jesus et al. 2007) | |
| 17, 29–32 (aqueous buffer soluble total lysate) | Sera from naturally infected dogs and mice | T. gondii; cross-reactivity with 45–50, 60, 82, 88, 97 and 120 kDa antigens as determined by immunoblot inhibition assays | (Silva et al. 2007) | |
| Reduced | 17, 31, 34, 37, 40·5, 47, 50, 55, 65 (SDS–PAGE sample buffer soluble total lysate) | Hyperimmune serum from a rabbit | Not examined | (Barta and Dubey, 1992) |
| 14, 20, 31, 35, 42, 54, 65, 76, 116 (aqueous buffer soluble total lysate) | Sera of cattle naturally or experimentally infected with N. caninum, T. gondii, S. cruzi, S. hirsuta, S. hominis | T. gondii, S. hirsuta: 14, 31, 37, 42, 55, 80 kDa recognized by anti-T. gondii; 11, 25, 34, 37 kDa recognized by anti-S. hirsuta serum. | (Baszler et al. 1996) | |
| 36·5–38, 45·5–48·5, 52–53·5, 58, 58·5, 59·5, 60·5, 62, 63·5, 64, 66·5, 67, 67·5, 68·5, 69·5 (total lysate antigen) | N. caninum or T. gondii experimentally infected cattle, sheep and goats | T. gondii; 63·5, 64, 66·5, 67, 67·5, 68·5, 69·5 kDa antigens recognized | (Harkins et al. 1998) | |
| 17–18, 34–35, 37, 60–62 (aqueous buffer soluble total lysate) | Sera from naturally infected pregnant cattle and aborted fetuses | Not examined | (Alvarez-Garcia et al. 2002) | |
| 18, 25, 33, 35–36, 45–46, 47, 60–62 (SDS–PAGE sample buffer soluble total lysate) | Sera from naturally infected bovines | Not examined | (Okeoma et al. 2004) |
a
Cross-reacting immunodominant antigen bands underlined.